Figure 5 Expression of SIK3, PSD-95, and proteins associated with tau modification before administration of inhibitor or agonist in rats(A) Compared with the Control group, the expression level of tau protein in the hippocampus in the SD group was upregulated. Tau protein phosphorylation’s expression level was significantly upregulated, with a decreased acetylation level and an increased phosphorylation/acetylation ratio. The Sevo group exhibited increased SIK3 expression, while other changes were similar to those in the SD group. There was no statistically significant difference between the 2 groups. Compared with the SD group, the expression of SIK3 in the SD+Sevo group was upregulated. The levels of tau and its phosphorylation were increased, and the phosphorylation/acetylation ratio was increased, with statistical significance. In the change of PSD-95, the expression of the SD+Sevo group was significantly reduced. Simultaneously, the SD group and Sevo group also showed a certain degree of expression decline, and the difference was statistically significant. (* P<0.05, Control vs SD, Control vs Sevo, SD vs SD+Sevo). (B) Compared with the Con group, the expressions of SIK3, tau, p-tau were slightly increased, and ace-tau expression was slightly decreased in the SD group. The expression levels of related proteins in the Prop group were unchanged compared with the Con group. SIK3, tau, and p-tau were expressed at reduced levels. The phosphorylation/acetylation ratio was decreased in the SD+Prop group compared with the SD group. Their expression levels were similar to those in the Con group. For PSD-95 expression, SD reduced its expression, and there were no significant differences in PSD-95 expression between Con, Prop, and SD+Prop groups. (* p<0.05, Con vs SD, Con vs Prop, SD vs SD+Prop).