10 February 2021: Lab/In Vitro Research
Knockdown of Dual Oxidase 1 (DUOX1) Promotes Wound Healing by Regulating Reactive Oxygen Species (ROS) by Activation of Nuclear Factor kappa B (NF-κB) Signaling
Hui Wang 1BC* , Haowei Lu 2DF* , Yige Wu 1AE*DOI: 10.12659/MSM.926492
Med Sci Monit 2021; 27:e926492
Figure 1 DUOX1 was highly expressed in slow-healing granulation tissue of burn wounds (A) Clinical burned wounds with fast healing (n=12) and slow healing (n=12) granulation tissue and normal tissue (n=6) were collected, and Q-PCR was used to detect the expression of DUOX1. Primary fibroblasts were isolated from fast-healing, slow-healing, and normal tissues. (B) Collagen I and III protein levels were detected by Western blotting. (C) Cell proliferation was detected by CCK8 at 0, 24, 48, and 72 h. * P<0.05, ** P<0.01, *** P<0.001 vs normal; # P<0.05, ## P<0.01 vs slow-healing. Normal tissues: skin tissue taken from the surface of traumatic injury, fast-healing granulation tissue: better recovery after 14 days’ treatment, slow-healing (n=12) granulation tissue: poor recovery after 14 days’ treatment.