H-Index
73
Scimago Lab
powered by Scopus
JCR
Clarivate
Analytics
call: +1.631.470.9640
Mon-Fri 10 am - 2 pm EST

Logo



eISSN: 1643-3750


Published: 2019-02-22

Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Proliferation and Metastasis of Osteosarcoma Cells by Targeting c-Met and SOX4 via miR-34a/c-5p and miR-449a/b

Zhenyu Sun, Tianfang Zhang, Bo Chen

(Department of Hand Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China (mainland))

Med Sci Monit 2019; 25:1410-1422

DOI: 10.12659/MSM.912703


BACKGROUND: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a functional long non-coding RNA involved in many biologic processes. The study was aimed to explore the functional roles of MALAT1 in osteosarcoma progression.
MATERIAL AND METHODS: A total of 76 osteosarcoma tissues and paired adjacent non-tumor tissues were collected from surgical resection. MALAT1, miRNAs, and genes mRNA expression levels were detected using quantitative real-time PCR (qRT-PCR). Protein expression level, cell proliferation, migration, and invasion were assessed using western blot, Cell Counting Kit-8 (CCK-8), wound-healing assay, and Matrigel invasion assay respectively. The target relationships among miRNAs, MALAT1, and mRNA were detected via dual-luciferase reporter assay.
RESULTS: We found that MALAT1 was frequently upregulated in osteosarcoma samples and cell lines and a high level of MALAT1 predicted poor overall survival in osteosarcoma patients. Knockdown of MALAT1 inhibited proliferation, migration, and invasion of osteosarcoma cells. Further study showed a positive correlation between MALAT1 and c-Met or SOX4 expression. Mechanistic investigations demonstrated that MALAT1, as a competing endogenous RNA (ceRNA), regulated osteosarcoma proliferation and metastasis through competitively binding to miR-34a/c-5p and miR-449a/b.
CONCLUSIONS: Taken together, our study illustrates a new regulatory mechanism for MALAT1 and may provide a novel therapeutic strategy for the treatment of osteosarcoma.

Keywords: MicroRNAs, Neoplasm Metastasis, Osteosarcoma



Related Articles (0)

Coming Soon...

Published: 2019-02-22

The C-Reactive Protein/Albumin Ratio and Complete Blood Count Parameters as Indicators of Disease Activit...


Nurten Seringec Akkececi, Gozde Yildirim Cetin, Hasan Gogebakan, Can Acipayam

Med Sci Monit 2019; 25:1401-1409

DOI: 10.12659/MSM.912495

Published: 2019-02-21

Effect of 3D Printing Individualized Ankle-Foot Orthosis on Plantar Biomechanics and Pain in Patients wit...


Rui Xu, Zhonghan Wang, Tingjian Ma, Zhenxiao Ren, Hui Jin

Med Sci Monit 2019; 25:1392-1400

DOI: 10.12659/MSM.915045