H-Index
75
Scimago Lab
powered by Scopus
JCR
Clarivate
Analytics
21%
Acceptance
Rate
call: +1.631.470.9640
Mon-Fri 10 am - 2 pm EST

Logo



eISSN: 1643-3750

Get your full text copy in PDF

Upregulation of KIF26B, Cell Migration and Proliferation of Human Ovarian Cancer Cell Lines In Vitro, and Patient Outcomes from Human Bioinformatic Analysis

Xingshuang Yang, Lan Zhang, Lei Xie

(Department of Gynecology and Obstetrics, Linyi Central Hospital, Linyi, Shandong, China (mainland))

Med Sci Monit 2018; 24:3863-3872

DOI: 10.12659/MSM.907889


BACKGROUND: The aim of this study was to assess the expression profile of the KIF26B gene, its effects on ovarian cancer cell behavior in vitro, the clinical prognostic value of expression of the KIF26B gene and associated signaling pathways, from bioinformatics data analysis.
MATERIAL AND METHODS: The Cancer Genome Atlas-Ovarian Cancer (TCGA-OV) database and the online Kaplan-Meier plotter for ovarian cancer were analyzed. Human ovarian cancer cell lines A2780 and SKOV3 were used to study the in vitro effects of KIF26B gene expression on cell proliferation and cell invasion. Genes that interacted with, co-localized to, and were co-expressed with the KIF26B gene (Pearson’s r ≥0.6) were identified and underwent Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
RESULTS: Increased expression of the KIF26B gene increased the proliferation and migration of A2780 and SKOV3 cells in vitro. KIF26B protein expression was upregulated in ovarian cancer tissue and was associated with lymphatic and venous invasion. TCGA-OV data analysis showed that increased expression of the KIF26B gene was significantly associated with reduced 3-year, 5-year, and 10-year overall survival (OS), progression-free survival (PFS), and post-progression survival (PPS). The genes that interacted with, co-localized to, and were co-expressed with KIF26B were enriched in several KEGG pathways; upregulation of the KIF26B gene was associated with TGF-β signaling pathway.
CONCLUSIONS: Upregulation of KIF26B enhanced proliferation and migration of ovarian cancer cells in vitro. Bioinformatics analysis supported the association between increased expression of the KIF26B gene and reduced clinical outcome in patients with ovarian carcinoma.

This paper has been published under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially.
I agree