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Mingming Xue, Chaolumen Qiqige, Qi Zhang, Haixia Zhao, Liping Su, Peng Sun, Pengwei Zhao
(Department of Physiology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China (mainland))
Med Sci Monit 2018; 24:4433-4439
The aim of this study was to investigate the effects of TNF-α and IL-10 on the expression of ICAM-1 and CD31 in human coronary artery endothelial cells (HCAEC).
MATERIAL AND METHODS: HCAEC was treated with 0, 2.5 μg/l, 5 μg/l, and 10 μg/l of TNF-α for 2 h, 6 h, and 10 h, and with 0 μg/l, 10 μg/l, 100 μg/l, and 200 μg/l of IL-10 for 5 h, 10 h and 15 h, respectively. RNA inference of TNF-αR was performed with siRNA. Real-time PCR, Western blot analysis, and ELSA were used to detect the mRNA level and protein level of ICAM-1 and CD31.
RESULTS: TNF-α significantly increased the mRNA and protein expression of ICAM-1 (P<0.05), and 2.5 μg/l TNF-α had the most obvious effect. RNAi of TNF-aR reduced the induction of TNF-α on the mRNA and protein expression of ICAM-1 (P<0.05). TNF-α significantly decreased the CD31 in the supernatant (P<0.05), and 2.5 μg/l TNF-a had the most obvious effect. IL-10 significantly decreased the ICAM-1 protein level. IL-10 decreased the mRNA expression and the protein expression of CD31. The effect on mRNA was not significant (P>0.05), while the effect on the protein expression was significant (P<0.05).
CONCLUSIONS: TNF-α and IL-10 treatment can affect the expression of ICAM-1 and CD31 in HCAEC.