24 August 2017 : Laboratory Research
IL-17A Inhibits Osteogenic Differentiation of Bone Mesenchymal Stem Cells via Wnt Signaling Pathway
Zhenguo Wang1ABCE, Ying Jia1ABCDE*, Fu Du2BCD, Min Chen1ABF, Xiuhua Dong1ACF, Yan Chen1BDE, Wen Huang3BEDOI: 10.12659/MSM.903027
Med Sci Monit 2017; 23:4095-4101
Abstract
BACKGROUND: Interleukin-17A (IL-17A) is not only an important modulator of inflammatory reactions, but also affects bone metabolism, which is involved in osteogenic differentiation of stem cells. However, the role and mechanism of IL-17A in osteogenic differentiation of bone mesenchymal stem cells (BMSCs) are not fully understood. In this study, we investigated the role and mechanism of IL-17A in osteogenic differentiation of BMSCs.
MATERIAL AND METHODS: The osteogenic differentiation of BMSCs was induced by osteoblast-induction medium with IL-17A or without IL-17A. The osteogenic differentiation of BMSCs was confirmed by the alkaline phosphatase and alizarin red staining. The lentiviral plasmid was used to construct the sFRP1-shRNA expression vector. The associated osteogenic differentiation marks (RUNX2, ALP, OPN), Wnt signaling pathway inhibitor (sFRP1), and modulators of Wnt signaling pathway (Wnt3, Wnt6) were detected by qRT-PCR and Western blot method.
RESULTS: The results showed that the addition of IL-17A inhibited osteogenic differentiation of BMSCs. IL-17A induced up-regulated expression of sFRP1 and down-regulated expression of Wnt3 and Wnt6 in BMSCs. In addition, sFRP1-shRNA abolished the inhibition effect of IL-17A in osteogenic differentiation of BMSCs and induced up-regulated expression of Wnt3 and Wnt6 in the Wnt signaling pathway in BMSCs.
CONCLUSIONS: Our findings show that IL-17A inhibits osteogenic differentiation of bone mesenchymal stem cells via the Wnt signaling pathway.
Keywords: Receptors, Interleukin-17
Editorial
01 July 2026 : Editorial
Editorial: The WHO Identifies Ebola Disease Due to Bundibugyo Virus as a Public Health Emergency of International Concern (PHEIC) as Vaccine Development Accelerates
DOI: 10.12659/MSM.954627
Med Sci Monit 2026; 32:e954627
In Press
Clinical Research
Association Between Total Cholesterol–to–High-Density Lipoprotein Ratio and Gestational Hypertension: A Cas...Med Sci Monit In Press; DOI: 10.12659/MSM.952395
Review article
Clinical Use of Endotracheal Intubation Without Neuromuscular Blockade: The Current Stage of KnowledgeMed Sci Monit In Press; DOI: 10.12659/MSM.951765
Clinical Research
Effect of Dexmedetomidine Hydrochloride Nasal Spray on Anxiety and Sleep in Patients Undergoing Gynecologic...Med Sci Monit In Press; DOI: 10.12659/MSM.952465
Clinical Research
Prognostic Value of Mortality Scoring Systems in Patients With Severe Burns: Identifying Key Predictors of ...Med Sci Monit In Press; DOI: 10.12659/MSM.951713
Most Viewed Current Articles
17 Jan 2024 : Review article 14,176,173
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
13 Nov 2021 : Clinical Research 3,757,769
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
14 Dec 2022 : Clinical Research 2,466,143
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research 708,797
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387