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Chun-Yan Wang, Shu-Guang Wang, Jia-Li Wang, Li-Ying Zhou, Hong-Jun Liu, Yi-Feng Wang
(Department of Obstetrics and Gynecology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China (mainland))
Med Sci Monit 2016; 22:3514-3522
The aim of this study was to investigate the possible associations of miRNA-27a and Leptin polymorphisms with the risk of recurrent spontaneous abortion (RSA).
MATERIAL AND METHODS: Between May 2013 and April 2015 at Shenzhen Longhua New District Central Hospital, we randomly recruited 138 RSA patients as the case group and another 142 normal pregnancy women as the control group. We used denaturing high-performance liquid chromatography (DHPLC) to determine the genotypes and allele frequencies of miRNA-27a rs895819 A/G and Leptin rs7799039 G/A.
RESULTS: The GG genotype and G allele frequencies of miRNA-27a rs895819 A/G were higher in the case group than in the control group, and the AA genotype and A allele frequencies of Leptin rs7799039 G/A were also higher in the case group than in the control group (all P<0.05). MiRNA-27a rs895819 A/G and Leptin rs7799039 G/A polymorphisms increased the risk of RSA (Exp (B)=2.732, 95% CI=1.625~4.596, P=0.000; Exp (B)=4.081, 95% CI=1.817~9.164, P=0.001). GG-AA or AG-AA carriers had a higher risk of RSA. The miRNA-27a expression of AA carriers of miRNA-27a rs895819 was lower than that of AG+GG carriers both in the case and control groups (all P=0.024). The plasma leptin concentration of GG carriers was lower than that of GA+AA carriers in the case group (P=0.026).
CONCLUSIONS: The polymorphisms of miRNA-27a rs895819 A/G and Leptin rs7799039 G/A may contribute to an increased risk of RSA.
Keywords: Adult, Abortion, Habitual - genetics, Base Sequence, Chromatography, High Pressure Liquid, Gene Expression Regulation, Gene Frequency - genetics, Genetic Loci, Genetic Predisposition to Disease, Leptin - genetics, Logistic Models, MicroRNAs - genetics, Polymorphism, Single Nucleotide - genetics, Pregnancy, Risk Factors