H-Index
85
Scimago Lab
powered by Scopus
JCR
Clarivate
Analytics
12%
Acceptance
Rate
call: +1.631.470.9640
Mon-Fri 10 am - 2 pm EST

Logo

Medical Science Monitor Basic Research
AmJCaseRep

Annals
ISI-Home

eISSN: 1643-3750

Get your full text copy in PDF

Hyperbaric Oxygen Alleviates Secondary Brain Injury After Trauma Through Inhibition of TLR4/NF-κB Signaling Pathway

Xiang-En Meng, Yu Zhang, Na Li, Dan-Feng Fan, Chen Yang, Hang Li, Da-Zhi Guo, Shu-Yi Pan

(Department of Hyperbaric Oxygen, Navy General Hospital, Beijing, China (mainland))

Med Sci Monit 2016; 22:284-288

DOI: 10.12659/MSM.894148


BACKGROUND: The aim of this study was to investigate the efficacy of hyperbaric oxygen in secondary brain injury after trauma and its mechanism in a rat model.
MATERIAL AND METHODS: A rat model of TBI was constructed using the modified Feeney’s free-fall method, and 60 SD rats were randomly divided into three groups – the sham group, the untreated traumatic brain injury (TBI) group, and the hyperbaric oxygen-treated TBI group. The neurological function of the rats was evaluated 12 and 24 hours after TBI modeling; the expression levels of TLR4, IκB, p65, and cleaved caspase-3 in the peri-trauma cortex were determined by Western blot; levels of TNF-α, IL-6, and IL-1β were determined by ELISA; and apoptosis of the neurons was evaluated by TUNEL assay 24 hours after TBI modeling.
RESULTS: Hyperbaric oxygen therapy significantly inhibited the activation of the TLR4/NF-κB signaling pathway, reduced the expression of cleaved caspase-3, TNF-α, IL-6 and IL-1β (P<0.05), reduced apoptosis of the neurons and improved the neurological function of the rats (P<0.05).
CONCLUSIONS: Hyperbaric oxygen therapy protects the neurons after traumatic injury, possibly through inhibition of the TLR4/NF-κB signaling pathway.

Keywords: Apoptosis - drug effects, Animals, Brain Injuries - therapy, Caspase 3 - metabolism, Cytokines - metabolism, Hyperbaric Oxygenation, I-kappa B Proteins - metabolism, In Situ Nick-End Labeling, NF-kappa B - metabolism, Neurons - pathology, Oxygen - pharmacology, Rats, Sprague-Dawley, Signal Transduction - drug effects, Toll-Like Receptor 4 - metabolism, Transcription Factor RelA - metabolism

This paper has been published under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially.
I agree