Get your full text copy in PDF
Xin Wang, Jianming Wei, Lijian Pan, Yijun Shi, Haihong Lin, Hui Gong
(Division of Cardiology, Department of Medicine, Jinshan Hospital, Fudan University, Shanghai, China (mainland))
Med Sci Monit 2015; 21:1494-1499
The aim of this study was to investigate the effects of arginine in the development of atherosclerosis in rats fed a high-fat diet supplemented with arginine and to evaluate the role of CD36 in this process.
Material and Methods: A total of 40 Sprague-Dawley rats were randomly assigned to 4 groups: control group, fat diet group, simvastatin group, and arginine group. They were fed for 12 weeks and were then sacrificed. Immunohistochemical CD36 expression and pathology was investigated in the aorta; CD36 expression in mononuclear cells was detected by Western blot and RT-PCR.
Results: The thickness of the aortal intima, media, and I/M significantly decreased in the arginine group rats compared with those in the fat diet group (P<0.05). CD36 expression was up-regulated in rats in the fat diet group compared with the control group and was down-regulated in rats in the arginine group compared with rats in the fat diet group.
Conclusions: The addition of arginine has a significant effect on reducing rat atherosclerosis development, which may be attributed to both the down-regulation of CD36 expression in rat aortic endothelial and blood mononuclear cells and the NO pathway.
Keywords: Antigens, CD36 - metabolism, Animals, Aorta - pathology, Arginine - pharmacology, Atherosclerosis - prevention & control, Blotting, Western, Diet, High-Fat - adverse effects, Dietary Supplements, Rats, Reverse Transcriptase Polymerase Chain Reaction, Statistics, Nonparametric, Tunica Intima - pathology