H-Index
75
Scimago Lab
powered by Scopus
JCR
Clarivate
Analytics
17%
Acceptance
Rate
call: +1.631.4701.9640
Mon-Fri 10 am - 2 pm EST

Logo



eISSN: 1643-3750

Get your full text copy in PDF

Using a novel in vivo model to study the function of nuclear factor kappa B in cerebral ischemic injury

Rui Wang, Songlan Liang, Hui Yue, LIjie Chen

Med Sci Monit 2012; 18(11): BR461-467

DOI: 10.12659/MSM.883539


Background:    Cerebral ischemia is a situation with a deficit blood supply to the brain, which eventually leads to cell death, inflammation, and tissue damage. Nuclear factor kappa B (NF-kappaB) plays an important role in inflammation and immune regulation. The aim of this study was to test the function of the activation of NF-kappaB in vivo in cerebral ischemic injury.
    Material/Methods:    We generated an animal model that used the method of occlusion of the middle cerebral artery (MCAO). The 60 traits were equally divided into 5 groups to investigate the role of NAC pretreatment: (1) sham-operation (control), (2) ischemia for 6 hours, (3) ischemia for 6 hours and NAC pretreatment, (4) ischemia for 24 hours, (5) ischemia for 24 hours and NAC pretreatment. The 36 rats were divided randomly into 3 groups: (A) recombinant adenovirus expressing wild-type kappaBalpha (AdIkappaBalphaM) group, (B) recombinant adenovirus expressing wild-type IkappaBalpha (AdIkappaBalpha) group, and (C) simple ischemia group. Triphenyltetrazolium chloride (TTC) was used to measure infarct volume. Detection of expression of NF-kappaB was by Immunohistochemistry analysis.
    Results:    The infarct size of the 24-hours ischemia groups were bigger than those of 6-hours ischemia groups (P<0.01). The infarct size of using NAC pretreatment groups was obviously reduced compared with saline control groups (P<0.01).The percentage of cortical p65-positive cells of the group of (A) were significantly less than the groups of (B) and (C).
    Conclusions:    Our data suggest that N-acetylcysteine (NAC) and Ad-IkappaBalpha-Mut can inhibit the activation of NF-kappaB in vivo, reduce the focal infarct size, and protect the brain tissue in ischemia.

This paper has been published under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially.
I agree