30 December 2008
Ethanol inhibited apoptosis-related RNA binding protein, Napor-3 gene expression in the prenatal rat brain
Nibedita NahaABDEG, Hae Young LeeBCFG, Mohammad Imran NaserC, Tae Ju ParkF, Sung Hoon KimA, Myeong Ok KimAGMed Sci Monit 2009; 15(1): BR6-12 :: ID: 869518
Abstract
Background
Cell death and differentiations are the critical processes in developing fetal brain, where ethanol induces lots of changes in gene expression patterns of fetal nervous system leading to fetal alcohol syndrome (FAS). The objective of the present study was to observe whether maternal ethanol exposure can alter gene expression pattern in mother and in fetus during mid and late prenatal stage.
Material and Method
10% ethanol was orally applied to female Spraque-Dawley rats and fetuses were sacrificed on gestational day (GD) 19.5 and 21.5. Total mRNA was isolated for differential-display PCR (DD-PCR) and sequence was analyzed to find out the homologous gene(s) using GenBank database of the BLAST program. Finally, the gene expression pattern in different maternal and fetal brain areas of the control and the ethanol treated groups were studied by RNase protection assay (RPA) and in situ hybridization.
Results
Out of several differentially expressed genes, apoptosis-related RNA binding protein (RBP), 'Napor-3' mRNA expression was significantly inhibited by ethanol in fetal rat fore-, mid- and hind- brain, and adult rat cortex and hippocampus when compared with the untreated control. The cDNA analysis was further supported our result (accession: AF090697, 95% sequence homology).
Conclusions
The age and area dependent suppression of apoptosis-related RBP, Napor-3 gene expression in proliferating fetal brain by maternal ethanol suggesting high susceptibility towards ethanol intake at the time of neuronal cell development and proliferation. Further, ethanol also affects maternal brain tissues that may be one of the reasons for ethanol-induce irreversible damage of the developing brain. The present study for the first time provides the evidence that Napor-3 suppression by ethanol during mid and late stage fetal brain converts natural physiological event apoptosis into pathological process, which may be useful as a novel therapeutic approach towards FAS-associated developmental brain damage as a consequence of maternal drinking behavior.
Keywords: RNA-Binding Proteins - metabolism, Sequence Analysis, DNA, Nerve Tissue Proteins - metabolism, Molecular Sequence Data, Maternal Exposure - adverse effects, In Situ Hybridization, Gene Expression Regulation, Developmental - drug effects, Ethanol - toxicity, DNA, Complementary - genetics, Brain - metabolism, Base Sequence, Analysis of Variance
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
08 Mar 2024 : Animal Research
Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hyp...Med Sci Monit In Press; DOI: 10.12659/MSM.943443
18 Apr 2024 : Clinical Research
Comparative Analysis of Open and Closed Sphincterotomy for the Treatment of Chronic Anal Fissure: Safety an...Med Sci Monit In Press; DOI: 10.12659/MSM.944127
08 Mar 2024 : Laboratory Research
Evaluation of Retentive Strength of 50 Endodontically-Treated Single-Rooted Mandibular Second Premolars Res...Med Sci Monit In Press; DOI: 10.12659/MSM.944110
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952