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Factors influencing the cellular location of proteolytic enzymes of Bacillus intermedius

Margarita R. Sharipova, Evgeni V. Shakirov, Leila A. Gabdrakhmanova, Nelli P. Balaban, Natalia V. Kalachewa, Galina N. Rudenskaya, Inna B. Leshchinskaya

Med Sci Monit 2000; 6(1): BR8-12

ID: 509317

Thiol-dependent serine proteinase and glutamylendopeptidase of Bacillus intermedius 3-19 being prevailing enzymes in the total pool of extracellular proteinases (95%) of this microorganism in catalytic active form were detected on the membrane of the cells. Production of these enzymes was maximum on the medium containing inorganic phosphate and gelatin and decreased 2-4-fold on the medium with glucose and lactate. The level of the activity of extracellular enzymes correlated with that of corresponding membrane-bound proteins. The addition of CoCl2 (2 mM) into the medium caused essential increase in extracellular glutamylendopeptidase activity and promoted the release of membrane-bound enzyme into cultural fluid. Proteolytic activity was detected in cytoplasm also. Proteinases localized in cytoplasm were shown to differ in properties from those secreted.

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