01 November 1999
Native tetanus and pertussis toxins effect on electrical and chemical seizures in mice
Roman KorolkiewiczMed Sci Monit 1999; 5(6): BR1081-1089 :: ID: 503101
Abstract
The tetanus toxin (Tetx) administered intracerebroventricularly (i.c.v.) or intraperitoneally (i.p.) caused the death of 50% of experimental mice (LD50,) at a dose of 18.0 (11.5-28.2) MLDs or 11.0 (8.3-14.6) MLDs/kg each. The minimal lethal dose (MLD) is defined as the lowest dose of Tetx required to kill a 20.0 g albino mouse within 96 hours following i.p. treatment [24]. Tetx increased the convulsive threshold of the electrical current and diminished particular chemical convulsant potencies. At the same time Tetx increased the protection given by a number of anti-epileptics during maximal electroshock treatment (MES). There were no marked γ-aminobutyric acid (GABA) concentration and total L-glutamic acid decarboxylase activity changes(GAD) in whole-brain homogenates accompanying Tetx treatment. These results suggest the preponderance of inhibitory over excitatory transmission probably due to Tetx action at the neuronal level. Tetx that penetrates into the central nervous system (CNS) following an i.p. injection evokes changes similar to those noted during Tetx administration into the lateral ventricle. Thus, both routes of adminstration demonstrate comparable predictive values in describing Tetx-induced changes during convulsive thresholds. Pertussis toxins (0.5 µg/animal, i.c.v., 120 h prior to testing), enhanced the chemical convulsants potency and decreased the carbamazepine protection during MES. It remained without marked influence upon the action of diphenylhydantoin and diazepam. These observations suggest that the enhanced sensitivity towards a number of chemical convulsants, irrespective of their action possibly results from a functional suppression of the inhibitory transmission at receptors coupled with pertussis toxin sensitive G proteins, rather than due to direct action on the G protein linked excitatory neurotransmission. The attenuation of carbamazepine efficacy might result in the sum of adenosine receptor antagonist drug properties and transmission suppression at the level of adenosine receptors coupled with G proteins sensitive towards pertussis toxins. Both toxins did not influence the total plasma levels of any anti-epileptic drug. Thus, the observed interactions between Tetx/pertussis toxins and anti-epileptic drugs probably occur at the neuronal level.
Keywords: Electroshock, Pertussis Toxin, Tetanus Toxin
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
06 Mar 2024 : Clinical Research
Prevalence and Variation of Medical Comorbidities in Oral Surgery Patients: A Retrospective Study at Jazan ...Med Sci Monit In Press; DOI: 10.12659/MSM.943884
08 Mar 2024 : Clinical Research
Evaluation of Foot Structure in Preschool Children Based on Body MassMed Sci Monit In Press; DOI: 10.12659/MSM.943765
15 Apr 2024 : Laboratory Research
The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in OsteoarthritisMed Sci Monit In Press; DOI: 10.12659/MSM.943738
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952