Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 September 1998

Attempt to evaluate activation of peripheral blood lymphocytes (CD69) after gluten challenge in children with coeliac disease

Andrzej Marek, Maria Korzon, Tomasz Smiatacz, Jacek Brodzicki

Med Sci Monit 1998; 4(5): CR821-825 :: ID: 502018

Abstract

Both the phenomenon of activating immune system of alimentary tract (GALT- gut associated lymphoid tissue) after gluten challenge as well as the role of cellular immune response in the development of morphological changes of intestinal mucosa in children with coeliac disease are well known. The introduction of new laboratory techniques and flow cytometry in particular, as well as the use of monoclonal antibodies opened new possibilities in the studies upon correlation between processes taking place in GALT and the changes within peripheral blood lymphocytes. At the same time, a question emerged whether these studies will allow to reduce the frequency of biopsy of intestinal mucosa in children with malabsorption syndrome, especially after gluten challenge. The aim of our study was to assess the diagnostic value of changes in the expression of selected CD antigens on T cells in peripheral blood as a result of gluten challenge. The intensification of CD3, CD19 and CD69 expression on peripheral blood lymphocytes was investigated before and after introducing gluten into the diet of 13 children with coeliac disease and of 7 children with secondary coeliac syndrome. Monoclonal antibodies and flow cytometry were used. The results obtained varied greatly in both groups. It was found that T cells activation prevails in children with coeliac disease, while in the case of children with secondary coeliac syndrome, the tendency for B cells activation is dominant. These differences, however, are not statistically significant. It is likely that the inflow of T and B cells with CD69 expression to circulation which is intensified by gluten challenge, is compensated by equally intensive homing of the lymphocytes in the mucous membrane of alimentary tract. Thus, the assessment of the intensification of CD69 expression on T and B cells of peripheral blood is useful neither in the differentiation between coeliac disease and secondary malabsorption syndrome, nor in the determination of disease intensity.

Keywords: peripheral blood lymphocytes, CD69 antigen, coeliac disease, children

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

18 Mar 2024 : Clinical Research  

Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative Study

Med Sci Monit In Press; DOI: 10.12659/MSM.944136  

0:00

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750