01 September 1997
Anti-CD3 antibody induces histidine-decarboxylase gene expression in human lymphocytes
Zsuzsa Radvany, Zsuzsa Darvas, Csaba Szalai, Andrs FalusMed Sci Monit 1997; 3(5): BR619-623 :: ID: 501496
Abstract
Histamine, synthetized by histidine decarboxylase (HDC) has been found in many proliferating cells, including haematopoetic cells and activated murine spleen lymphocytes suggesting a role of this monoamine in T-lymphocyte maturation and proliferation, as well. In this study HDC gene expression and enzyme protein were examined in anti-CD3 treated (activated) human Th1 leukemic cell line (Jurkat) and in peripheral lymphocytes of human healthy adult individuals. HDC, g-IFN, actin mRNAs and HDC enzyme protein levels were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and by western blotting, respectively. The expression of HDC during the T cell activation was studied using anti-CD3 antibody for different incubation times. We have shown that both Jurkat and healthy adult peripheral lymphocytes have a low constitutive HDC gene expression which can be further stimulated by monoclonal antibody against CD3, a part of T lymphocyte receptor. There was a concomitant increase in the γ-IFN and a slight decrease in the actin expression. The detection of HDC protein by western blotting showed marked difference between the T cell line (Jurkat) and the healthy lymphocytes; the HDC protein was found in much higher amount in the leukemic cell line than in the healthy T cells proving the role of intracellular histamine in the malignant cell proliferation. Histamine generating enzyme (HDC) is stored not only in mast cells, but in many different tissues undergoing rapid proliferation and growth suggesting that histamine a ubiquitously occuring local hormone, can function as a growth factor and chemical mediator, too. HDC expression in activated T cells suggests that intracellular histamine has a prominent role of T-lymphocyte activation and, as a newly recognised intracrine/autocrine signal transduction system has close relation to the regulation of cell division.
Keywords: Histamine, cell growth, Histidine Decarboxylase, anti-CD3 antibody, Lymphocyte Activation, leukemic cell line
Editorial
01 March 2024 : Editorial
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-ThalassemiaDOI: 10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
In Press
21 Feb 2024 : Clinical Research
Potential Value of HSP90α in Prognosis of Triple-Negative Breast CancerMed Sci Monit In Press; DOI: 10.12659/MSM.943049
22 Feb 2024 : Review article
Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...Med Sci Monit In Press; DOI: 10.12659/MSM.943168
23 Feb 2024 : Clinical Research
A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...Med Sci Monit In Press; DOI: 10.12659/MSM.943732
26 Feb 2024 : Clinical Research
Predictive Value of Combined HbA1c and Neutrophil-to-Lymphocyte Ratio for Diabetic Peripheral Neuropathy in...Med Sci Monit In Press; DOI: 10.12659/MSM.942509
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952