Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 January 2001

Computer analysis of normal and basal cell carcinoma mast cells.

Paweł Janowski, Michał Strzelecki, Ewa Brzezińska-Błaszczyk, Anna Zalewska

Med Sci Monit 2001; 7(2): CR260-265 :: ID: 421151

Abstract

BACKGROUND: Basal cell carcinoma (BCC) is the most frequently observedneoplasm of the human skin. Mast cells are regarded as important cellular elements of all organs andtissues of the human body. They are involved in many physiological and pathological processes, includinginflammation and fibrotic changes observed in BCC. The aim of our study was to characterise in detailmast cells observed in BCC and normal skin on the basis of computer analysis techniques.
MATERIAL ANDMETHODS: Series of photographs obtained from histopathological biopsies of basal cell carcinomas from18 patients, stained specifically for mast cells with Astra blue and nuclear red, were employed for thestudy. Control group consisted of series of photographs from normal skin obtained from 18 persons whounderwent plastic surgery operations. All the photographs underwent digital computer analysis by meansof a number of subroutines written in Delphi developed at the Technical University of Łódź.
RESULTS:Specific staining allowed for thresholding the image and subsequently mast cells were visualised as blackobjects on a white background. Those objects were then analysed in detail i.e. their geometrical features(area, perimeter, maximal diameter) were calculated. The employed image analysis techniques allowed forthe differentiation and detailed characteristics of mast cells stained with Astra blue and nuclear red.
CONCLUSIONS: We observed that mast cells in BCC gathered mainly in fibrous stroma and were more numerous,had considerably larger area, longer perimeter and maximal diameter than normal skin mast cells.

Keywords: basal cell carcinoma, Mast Cells, computer analysis techniques

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

18 Mar 2024 : Clinical Research  

Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative Study

Med Sci Monit In Press; DOI: 10.12659/MSM.944136  

0:00

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750