Scimago Lab
powered by Scopus
call: +1.631.470.9640
Mon-Fri 10 am - 2 pm EST


Medical Science Monitor Basic Research


eISSN: 1643-3750

Get your full text copy in PDF

Effect of anti-oxidants, free radical quenchers and cyclo-oxygenase inhibitoron benzo(a)pyrene-induced suppression of human lymphocyte mitogenesis in vitro.

Undurti N Das

Med Sci Monit 2002; 8(6): BR205-207

ID: 420823

BACKGROUND: Benzo(a)pyrene (BP) is a known carcinogen and immunosuppressor.Activation of BP to its reactive intermediates is cyclo-oxygenase (COX) dependent and occurs via a freeradical dependent mechanism. The aim of this study was to know whether anti-oxidants, free radical quenchersand (COX) inhibitor, indomethacin, influence the suppression of human T cells induced by BP in vitro.MATERIAL/METHODS:Human lymphocytes, obtained from healthy human volunteers, were stimulated to proliferate using phytohaemagglutinin(PHA). The effect of vitamin E, SOD, catalase, mannitol, and COX-inhibitor, indomethacin, on BP-inducedsuppression of human T cell proliferation was evaluated.RESULTS: Except for catalase, a hydrogen peroxide(H2O2) radical quencher, none of the agents tested could inhibit BP-induced suppression of human T cellproliferation in vitro.CONCLUSIONS: BP-induced suppression of human T cell proliferation is probably,induced by increased production of H2O2. Prostaglandins and superoxide anion and hydroxyl radical donot seem to participate in BP-induced human T cell growth suppression.

This paper has been published under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially.
I agree