H-Index
75
Scimago Lab
powered by Scopus
JCR
Clarivate
Analytics
18%
Acceptance
Rate
call: +1.631.470.9640
Mon-Fri 10 am - 2 pm EST

Logo



eISSN: 1643-3750

Get your full text copy in PDF

Regulation of various genes in human leukocytes acutely exposed to morphine: expression microarray analysis

George B. Stefano, John D. Burrill, Sergei Labur, Julie Blake, Patrick Cadet

Med Sci Monit 2005; 11(5): MS35-42

ID: 16159


Background:Previous studies from our laboratory have identified a novel mu opiate receptor µ[sub]3[/sub], which is expressed in several different cell types and tissues including human vascular endothelial cells, leukocytes and neural tissues. This novel µ receptor is selective for the opiate alkaloid morphine, since this receptor does not bind other opioid peptides.Material/Methods:This report details the acute affects of morphine exposure (1 µg/ml) to human leukocytes by analyzing gene expression using microarrays (Applied Biosystems). Robust estimation of the median fold change was used to identify candidates for significantly differentially expressed genes. An independent experiment using four same sample arrays was used to test the algorithm and to confirm the calculated percentage of falsely significant genes.Results:Data obtained from this study demonstrate that acute morphine exposure differentially affected genes that are involved in immune function, signal transduction, cell adhesion, and apoptosis.Conclusions:Acute morphine exposure to human leukocytes results in specific and significant alterations in gene expression.

This paper has been published under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially.
I agree