09 September 2002
Cytotoxic effect of cyclosporin A alone and in combination with 2-chlorodeoxyadenosine against P388 murine leukemia in vivo
Grażyna Józefowicz-Okonkwo, Anna Szmigielska-Kapłon, Tadeusz RobakMed Sci Monit 2002; 8(9): BR373-377 :: ID: 13268
Abstract
Summary
Background: The purpose of our study was to determine the antileukemic effects of cyclosporin A (CsA) and its interaction with a new purine analogue, 2-chlorodeoxyadenosine (2-CdA, cladribine)
on P388 murine leukemia in vivo.
Material/methods: Mice were engrafted intraperitoneally (i.p.) with 106 P388 leukemia cells on day 0 of each step of the two-step experiment. All treatments were initiated on the next day (day 1) as daily i.p. injections and lasted for five consecutive days. In the first part of the experiment, designed to establish the antileukemic effect of CsA, the mice received 10, 15, 20, 25 or 30 mg/kg of CsA.
In the second part, where the interaction between CsA and 2-CdA was examined, the animals were administered CsA at a dose of 15 mg/kg, or 2-CdA at a dose of 20 mg/kg, or CsA combined with 2-CdA at the same doses as in monotherapy.
Results: CsA did not influence the survival time of mice with P388 leukemia at any dose. CsA was used for the interaction study at a dosage of 15 mg/kg, as this was best tolerated by the animals.
The mice treated with combination therapy using CsA and 2-CdA survived longer than those treated with 2-CdA monotherapy (p=0.00015).
Conclusion: Our study revealed that CsA alone does not increase the survival time of mice with P388 leukemia, but it augments the antileukemic effect of 2CdA.
Keywords: Antineoplastic Combined Chemotherapy Protocols - therapeutic use, Cladribine - administration & dosage, Cyclosporine - administration & dosage, Drug Synergism, Immunosuppressive Agents - administration & dosage, Neoplasm Transplantation, Neoplasms - drug therapy, Purines - metabolism, Time Factors, Tumor Cells, Cultured
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
15 Apr 2024 : Laboratory Research
The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in OsteoarthritisMed Sci Monit In Press; DOI: 10.12659/MSM.943738
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
08 Mar 2024 : Animal Research
Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hyp...Med Sci Monit In Press; DOI: 10.12659/MSM.943443
18 Apr 2024 : Clinical Research
Comparative Analysis of Open and Closed Sphincterotomy for the Treatment of Chronic Anal Fissure: Safety an...Med Sci Monit In Press; DOI: 10.12659/MSM.944127
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952