miRNA Profiling of Hungarian Regressive Wilms’ Tumor Formalin-Fixed Paraffin-Embedded (FFPE) Samples by Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)

Gergely Buglyó; Zsófia Magyar; Éva Romicsné Görbe; Rita Bánusz; Monika Csóka; Tamás Micsik; Márta Mezei; Jaxi Ayman Shawky Yani; Péter Varga; Zoltán Sápi; Bálint Nagy

doi:10.12659/MSM.932731

05 October 2021: Clinical Research

miRNA Profiling of Hungarian Regressive Wilms’ Tumor Formalin-Fixed Paraffin-Embedded (FFPE) Samples by Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)

Gergely Buglyó ^1ABCDEF** , Zsófia Magyar ^2ABCD* , Éva Romicsné Görbe ^2AB , Rita Bánusz ^3AB , Monika Csóka ^3B , Tamás Micsik ^4BDE , Márta Mezei ^1BC , Jaxi Ayman Shawky Yani ^1BC , Péter Varga ^2A , Zoltán Sápi ^4A , Bálint Nagy ^1ACD

DOI: 10.12659/MSM.932731

Med Sci Monit 2021; 27:e932731

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Table 3 Logarithmic fold changes (log2FC) of miR-128-3p, miR-184, miR-194-5p, and miR-203a in Patient 1 (determined by a PCR array), Patients 2 to 8 (determined by individual primers using the median value of 3 technical triplicates), and mean.

	Patient 1 (array)	Patient 2	Patient 3	Patient 4	Patient 5	Patient 6	Patient 7	Patient 8	Mean fold change
miR-128-3p	3.21	−4.22	−1.35	−2.17	1.11	−0.25	0.79	4.32	0.18
miR-184	−3.71	−8.51	−1.77	−8.82	−5.97	−9.05	−6.14	2.54	−5.18
miR-194-5p	−6	−4.46	−3.69	−7.63	−4.04	−1.34	−6.15	−1.01	−4.29
miR-203a	−1.98	−3.68	−4.32	−3.47	−4.8	−0.29	−6.37	3.49	−2.68
Patient 8 was the one showing unusual histology (see and main text).

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miRNA Profiling of Hungarian Regressive Wilms’ Tumor Formalin-Fixed Paraffin-Embedded (FFPE) Samples by Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)

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