24 October 2021: Clinical Research
Vitamin D Receptor Is a Sepsis-Susceptibility Gene in Chinese Children
Danni He 12BCDEF* , Xiuxiu Lu 3ABCDEFG* , Wei Li 3BG , Yuanyuan Wang 4BD , Ning Li 3BC , Yuanmei Chen 2CEG , Lipeng Zhang 25EF , Wenquan Niu 1AEF* , Qi Zhang 2AEG*DOI: 10.12659/MSM.932518
Med Sci Monit 2021; 27:e932518
File 2 Primers involved in the experiment.
| Rs number | Primer name | Primer sequences | Tm (°C) | Product length (bp) |
|---|---|---|---|---|
| rs9729 | VDR-P1-F | CCTTGCACCTGCATCCGTAG | 60 | 856 |
| VDR-P1-R | GAAAAGGACACCGGACCATGA | |||
| rs2107301 | VDR-P2-F | CTGTGCCGTTCATTTGGA | 60 | 284 |
| VDR-P2-R | AGTGTTGGGCTGTCTGGT | |||
| rs2189480 | VDR-P3-F | AGAGAGCAGCTGAGGCAATG | 60 | 415 |
| VDR-P3-R | GGACACCATTACGCTCTGGA | |||
| rs2239185 | VDR-P4-F | TCATTGCCATTTCCATAC | 60 | 387 |
| VDR-P4-R | GACATTTACACCCTCCTCT | |||
| rs3782905 | VDR-P5-F | GACAGATGGTCCTTTCTT | 58 | 693 |
| VDR-P5-R | AATCCACTACCCACTACA | |||
| rs4516035 | VDR-P6-F | GATGGCTGCGGAAAACTCAC | 60 | 470 |
| VDR-P6-R | ATTGAGTTGTGAGGGGCTGG | |||
| rs7139166 | VDR-P7-F | AGGCATAGCGTTTGATTG | 58 | 212 |
| VDR-P7-R | GGTATTGGTGGTTGGAAA | |||
| rs11168266 | VDR-P8-F | TTTCACCATAGCAAACCCAA | 60 | 391 |
| VDR-P8-R | CTCCCAGCAGGCAGACAT | |||
| rs11168293 | VDR-P9-F | ACCAAGGAACCCTGAGAC | 60 | 481 |
| VDR-P9-R | GAAGGCAAATAGGAAACAAT |