08 September 2021: Animal Study
Pseudolaric Acid B Attenuates High Salt Intake-Induced Hypertensive Left Ventricular Remodeling by Modulating Monocyte/Macrophage Phenotypes
Fang-Fang Yu 1BE* , Guo-Hong Yang 1AG** , Shao-Bo Chen 1FG , Xiu-Long Niu 1F , Wei Cai 1CF , Yan-Yan Tao 1C , Xiu-Juan Wang 1BC , Ming Li 1BF , Yu-Ming Li 2DG , Ji-Hong Zhao 3AD*DOI: 10.12659/MSM.932404
Med Sci Monit 2021; 27:e932404
Table 1 List of primers used for the PCR analysis.
| Gene | Primers | Sequence | Expected amplicon (bp) |
|---|---|---|---|
| β-actin | Forward | 5′-CTAAGGCCAACCGTGAAAAG-3′ | 104 bp |
| Reverse | 5′-ACCAGAGGCATACAGGGACA-3′ | ||
| TNF-α | Forward | 5′-CTCCAGCTGGAAGACTCCTCCCAG-3′ | 171 bp |
| Reverse | 5′-CCCGACTACGTGCTCCTCACC-3′ | ||
| CCL-2 | Forward | 5′-TTAAGGCATCACAGTCCGAG-3′ | 129 bp |
| Reverse | 5′-TGAATGTGAAGTTGACCCGT-3′ | ||
| IL-1β | Forward | 5′-AACGTGTGGGGGATGAATTG-3′ | 130 bp |
| Reverse | 5′-CATACTCATCAAAGCAATGT-3′ | ||
| CCL-5 | Forward | 5′-CCATATGGCTCGGACACCACTC-3′ | 167 bp |
| Reverse | 5′-CACTTCTTCTCTGGGTTGGCAC-3′ | ||
| Ym-1 | Forward | 5′-CTGAACCTCGGGGGTTAGTA-3′ | 150 bp |
| Reverse | 5′-GAAGGAAGGAAAGGAGGGAG-3′ | ||
| Arg-1 | Forward | 5′-AGGAGAAGGCGTTTGCTTAG-3′ | 115 bp |
| Reverse | 5′-AGGAGAAGGCGTTTGCTTAG-3′ | ||
| MRC-1 | Forward | 5′-CATGAGGCTTCTCCTGCTTCT-3′ | 143 bp |
| Reverse | 5′-TTGCCGTCTGAACTGAGATGG-3′ | ||
| IL-10 | Forward | 5′-TGCACTACCAAAGCCACAAGG-3′ | 150 bp |
| Reverse | 5′-TGGGAAGTGGGTGCAGTTATTG-3′ | ||
| Arg-1 – arginase-1; CCL-2 – CC class chemokine ligand-2; CCL-5 – CC class chemokine ligand-5; IL-1β – interleukin-1β; IL-10 – interleukin-10; MRC-1 – mannose receptor-1; PCR – polymerase chain reaction; TNF-α – tumor necrosis factor-α; Ym-1 – chitinase3-like-1. | |||