20 August 2021: Animal Study
Knockout of the Cannabinoid Receptor 2 Gene Promotes Inflammation and Hepatic Stellate Cell Activation by Promoting A20/Nuclear Factor-κB (NF-κB) Expression in Mice with Carbon Tetrachloride-Induced Liver Fibrosis
Cuizhen Long 123ABCDEF* , Na Xie 12ABCDEF* , Yuanhui Shu 12ABCF , Yafeng Wu 14ABCF , Ping He 12BC , Yan Zhou 12BC , Yining Xiang 5CD , Junying Gu 12CD , Lei Yang 12DF , Yuping Wang 12ABCDEFG*DOI: 10.12659/MSM.931236
Med Sci Monit 2021; 27:e931236
Table 1 The primer sequences of CB2 gene used for PCR.
| Primers type | Sequence 5′-3′ |
|---|---|
| Mutant forward | GGGGATCGATCCGTCCTGTAAGTCT |
| Common reverse | GACTAGAGCTTTGTAGGTAGGCGGG |
| Wild-type forward | GGAGTTCAACCCCATGAAGGAGTAC |
| Three primers were simultaneously added to the PCR reaction system. Mutant forward and common reverse sequences can amplify 550 bp DNA products, common reverse and wild-type forward sequences can amplify 385 bp DNA products. | |