23 June 2021: Clinical Research
A Comparison of Detection in Sputum by the Conventional Culture and Fluorescent Polymerase Chain Reaction Methods
Xiaoqun Xu 12BD* , Huoyang Lv 3AE* , Fengwei Zhang 12DF , Houyong Zhu 4CDE* , Long Cai 12AE*DOI: 10.12659/MSM.930293
Med Sci Monit 2021; 27:e930293
Table 4 Detection conditions of fluorescent PCR.
| Stage | Temperature | Time | Cycle number | Other parameter settings |
|---|---|---|---|---|
| Stage 1 | 50°C | 2 min | 1 | Detection of fluorescein: FAM/TAMRA |
| Stage 2 | 95°C | 10 min | 1 | Reference fluorescence: ROX |
| Stage 3 | 95°C | 15 sec | 40 | Reaction system: 20 μL |
| 56°C | 30 sec | Fluorescence signal collection: Stage 3 56°C 30 sec |