23 June 2021: Clinical Research
A Comparison of Detection in Sputum by the Conventional Culture and Fluorescent Polymerase Chain Reaction Methods
Xiaoqun Xu 12BD* , Huoyang Lv 3AE* , Fengwei Zhang 12DF , Houyong Zhu 4CDE* , Long Cai 12AE*DOI: 10.12659/MSM.930293
Med Sci Monit 2021; 27:e930293
Table 3 Fluorescent PCR detection reaction system.
| PCR reaction solution | Primer | Probe | Sterilized purified water | Sample/ reference | Total volume |
|---|---|---|---|---|---|
| 10 μL | 1 μL | 0.5 μL | 4.5 μL | 4 μL | 20 μL |