23 June 2021>: Clinical Research
A Comparison of Detection in Sputum by the Conventional Culture and Fluorescent Polymerase Chain Reaction Methods
Xiaoqun Xu 12BD* , Huoyang Lv 3AE* , Fengwei Zhang 12DF , Houyong Zhu 4CDE* , Long Cai 12AE*DOI: 10.12659/MSM.930293
Med Sci Monit 2021; 27:e930293
Table 2 The universal nucleic acid detection kit.
Kit | Components | Storage conditions | Specifications and quantities | Main components |
---|---|---|---|---|
A-box | Primers | −20°C | 22 μL ×1 Tube | Upstream and downstream primers for detection |
Probe | −20°C, protect form light | 11 μL×1 Tube | Probe for detection | |
Negative control | −20°C | 15 μL ×1 Tube | Plasmid containing unrelated gene | |
Positive control | −20°C | 15 μL ×1 Tube | Plasmid Containing Target Gene | |
B-box | PCR reaction solution | 2–8°C, freeze prohibited | 220 μL ×1 Tube | dNTP (containing dUTP), Taq enzyme, UNG enzyme, reference fluorescence, MgCl2, buffer |