18 April 2021>: Animal Study
Left Ventricular Impaired Relaxation and Interstitial Myocarditis Identified in Sepsis-Associated Cardiac Dysfunction: Use of a Rodent Model
David J. Sturgess 1ABCDEFG* , Shannon Morrison 1BCDE , Brian Haluska 2BCD , Glenda C. Gobe 23BCDEG , Mark A. Jones 4ACDE , Sonia Volante 1BE , Bala Venkatesh 2ACDEGDOI: 10.12659/MSM.929512
Med Sci Monit 2021; 27:e929512
Table 3 Immunohistochemistry for monocyte/macrophage ED1 antibody in LPS model.
Group | No. ED1+ macrophages/monocytes | ||||
---|---|---|---|---|---|
<5 | 5–25 | 25–50 | 50–75 | Total | |
Control (no.) | 4 | 2 | 1 | 0 | 7 |
(%) | 57.14 | 28.57 | 14.29 | 0.00 | |
Endotoxin (no.) | 0 | 2 | 3 | 2 | 7 |
(%) | 0.00 | 28.57 | 42.86 | 28.57 | |
Total | 4 | 4 | 4 | 2 | 14 |
Staining for macrophages (ED1) demonstrated increased numbers in the ventricular myocardium of the study group. Five out of 7 endotoxic animals, and no control animals, demonstrated >25 macrophages in at least two out of ten high powered fields (P=0.0095; Cochrane-Armitage test). |