07 March 2021: Clinical Research
Association of Leptin Receptor Gene Polymorphisms with Keloids in the Chinese Han Population
Jing Liu 1ABCD , Limin Cai 1CDEF , Zepeng Zhang 1BCD , Yanli Ma 1BCDF , Yongchen Wang 2ADE*DOI: 10.12659/MSM.928503
Med Sci Monit 2021; 27:e928503
Table 1 Primer sequences of the studied LEPR SNPs.
| SNP | Alleles | MAF (1000g_CHBS) | iMLDR primers sequences |
|---|---|---|---|
| rs1137101 | G/A | 0.13 | F: 5′-TGTGCCAACAGCCAAACTCAAC-3′R: 5′-AGAAGCCACTCTTAATACCCCCAGT-3′ |
| rs1938496 | C/T | 0.21 | F: 5′-TGCTTGCCATTATGAAGAACAGC-3′R: 5′-CAAGAATCTGTGGGCAAAAAGCTC-3′ |
| rs6588147 | G/A | 0.16 | F: 5′-CCAGCCACAAAGAGCAAGGTAGA-3′R: 5′-GGAGGGTCAAGAGGCACTGAGA-3′ |
| rs7555955 | G/A | 0.20 | F: 5′-TGTTTTTCCAAAAGACCTTTGACCA-3′R: 5′-TCACGGGATGTTATGTTTCTATTTTGA-3′ |
| LEPR – leptin receptor; SNP – single nucleotide polymorphism; MAF – minor allele frequency; 1000g_CHBS – minor allele frequencies from HapMap of Han Chinese in Beijing, China(CHB) or Human Genome Project; iMLDR – improved multiple ligase detection reaction; F – forward; R – reverse. | |||