16 November 2020>: Lab/In Vitro Research
Construction of a Mex3c Gene-Deficient Mouse Model to Study C-FOS Expression in Hypothalamic Nuclei and Observe Morphological Differences in Embryonic Neural Tube Development
Yong Du 12ABDE** , Quan Huo 3BCEF* , Ting Li 3BCD , Dongjun Sun 3BEF , Ting Sun 4BCD , Zhiguo Lu 3BEFDOI: 10.12659/MSM.927334
Med Sci Monit 2020; 26:e927334
Table 2 DNA cycling condition.
Step | Temp | Time | Cycles |
---|---|---|---|
Initial denaturation | 94°C | 3 min | |
Denaturation | 94°C | 30 s | 35× |
Annealing | 52°C | 35 s | |
Extension | 72°C | 35 s | |
Additional extension | 72°C | 5 min |