16 November 2020>: Lab/In Vitro Research
Construction of a Mex3c Gene-Deficient Mouse Model to Study C-FOS Expression in Hypothalamic Nuclei and Observe Morphological Differences in Embryonic Neural Tube Development
Yong Du 12ABDE** , Quan Huo 3BCEF* , Ting Li 3BCD , Dongjun Sun 3BEF , Ting Sun 4BCD , Zhiguo Lu 3BEFDOI: 10.12659/MSM.927334
Med Sci Monit 2020; 26:e927334
Table 1 PCR mixture.
Component | ×1 |
ddHO | 9.5 μl |
Product primer F1 | 1.0 μl |
Reverse primer R1 | 1.0 μl |
Premix Taq | 12.5 μl |
DNA | 1.0 μl |