03 November 2020: Lab/In Vitro Research
Role of Sevoflurane on Natural Killer Group 2, Member D-Mediated Immune Response in Non-Small-Cell Lung Cancer: An Study
Soeun Jeon 12ABCDEFG , Hae-Kyu Kim 1DE , Jae-Young Kwon 1DF , Seung-Hoon Baek 1DE , Hyun-Su Ri 3BC , Ho Jung Choi 45CD , Hae-Ryung Cho 45BD , Young Shin Lee 45BD , Joo-Young Kim 6BE , Jinsil Kim 1BF , Jaeho Bae 45ACDEF** , Hyeon-Jeong Lee 12ACDEF**DOI: 10.12659/MSM.926395
Med Sci Monit 2020; 26:e926395
Table 1 List of primer used in multiplex RT-PCR.
| Name | Polarity | Sequence (5′→3′) | Amplicon length (bp) |
|---|---|---|---|
| MICA | Sense | TTGAGCCGCTGAGAGGGTGGC | 460 |
| Anti-sense | GGGAGAGGAAGAGCTCCCCATC | ||
| MICB | Sense | GCCCCCTGACCCCTTGTTCC | 358 |
| Anti-sense | GGGCTGGTCAACTTGGCGAAA | ||
| ULBP1 | Sense | TGGCTGGTCCCGGGCAGGAT | 266 |
| Anti-sense | GAATGTCAAGCAGTTGCCCTTTAAGGAAA | ||
| ULBP2 | Sense | TCAAACTCGCCCTTCTGTCTGGC | 194 |
| Anti-sense | GCAGGAATTCCATCAGGTAGCACCA | ||
| ULBP3 | Sense | AGGTCTTATCTATGGGTCACCTAGAAG | 132 |
| Anti-sense | TGAAATCCTCCAGCTCAGTGTCAGC | ||
| MMP1 | Sense | AGACAAAGGCAAGTTGAAAAGCGGA | 195 |
| Anti-sense | TTGCTCCCAGCGAGGGTTCC | ||
| MMP2 | Sense | ACGGACTCCTGGCTCATGCC | 305 |
| Anti-sense | CTGTCCTTCAGCGTTGCCGC | ||
| MMP9 | Sense | CGACCCGAGCTGACTCGACG | 390 |
| Anti-sense | GCGGTGTGGTGGTGGTTGGA | ||
| RPL19 | Sense | ATGCTCAGGCTTCAGAAGAGGCTCG | 550 |
| Anti-sense | TGATGATCTCCTCCTTCTTGGCCTG | ||
| ACTB | Sense | TCCATCCTGGCCTCGCTGTC | 93 |
| Anti-sense | GCATTTGCGGTGGACGATGG | ||
| RT-PCR – reverse transcriptase-polymerase chain reaction; MICA/B – MHC class I chain-related molecules A/B; ULBP – UL16-binding proteins; MMP – matrix metallopeptidase; RPL19 – ribosomal protein L19; ACTB – β-actin. | |||