11 August 2020>: Lab/In Vitro Research
Regulates the Proliferation and Migration of H9c2 Cells
Hongshu Wang 1BCDEF* , Yong Liu 2BC* , Shen Han 1B , Yunfeng Zi 1B , Yayong Zhang 1B , Ruize Kong 3B , Zu Liu 1B , Zhibin Cai 1B , Chongbin Zhong 4B , Wei Liu 5B , Lifeng Li 4B , Lihong Jiang 3AG*DOI: 10.12659/MSM.925388
Med Sci Monit 2020; 26:e925388
Table 3 The protocol of quantitative reverse transcription-polymerase chain reaction (qRT-PCR) of miRNAs.
Temperature | Times | Cycle | |
---|---|---|---|
Pre-denature | 95°C | 10 min | 1 cycle |
Denature | 95°C | 10 s | |
Amplification | Tm-2°C | 20 s | 40 cycles |
72°C | 10 s | ||
Melting curves | 65–95°C | 0.5°C/time | 6 s/time |
30°C | 30 s |