07 May 2020: Lab/In Vitro Research
Potential Molecular Mechanisms and Drugs for Aconitine-Induced Cardiotoxicity in Zebrafish through RNA Sequencing and Bioinformatics Analysis
Mingzhu Wang 1CDEF , Yanan Shi 1BDF , Lei Yao 1CDF , Qiang Li 2ACDFG , Youhua Wang 3ABDFG , Deyu Fu 1ABEFG*DOI: 10.12659/MSM.924092
Med Sci Monit 2020; 26:e924092
Table 3 Validation the expression of CDK1 using different method and tissues.
| Genes | cdk1 | cdk1 | CDK1 | CDK1 |
|---|---|---|---|---|
| Tissue | ZebrafishOur data | Mus musculusGSE71906 | HumanGSE65705 | HumanGSE95140 |
| Methods | RNA-seq | RNA-seq | RNA-seq | Single-Cell RNA-seq |
| LogFC | −1.329081 | −0.130911 | −0.216376 | −0.071611 |
| Regulation | Down | Down | Down | Down |
| Comparing the myocardial injury tissue of mouse and human from the GEO database (GSE71906, GSE65705 and GSE95140) with the zebrafish heart injury in present study, the expression of CDK1 is all downregulated in 3 types of samples. GEO – Gene Expression Omnibus; CDK1 – cyclin-dependent kinase-1. | ||||