30 August 2020: Animal Study
Promoting Role of Long Non-Coding RNA Small Nucleolar RNA Host Gene 15 (SNHG15) in Neuronal Injury Following Ischemic Stroke via the MicroRNA-18a/CXC Chemokine Ligand 13 (CXCL13)/ERK/MEK Axis
Tiezhu Guo 1AB , Yueting Liu 2CE , Xinliang Ren 1DE , Wei Wang 1EF , Hanrui Liu 3F*DOI: 10.12659/MSM.923610
Med Sci Monit 2020; 26:e923610
Table 1 Primer sequences for RT-qPCR.
| Gene | Primer sequence (5′-3′) |
|---|---|
| SNHG15 | F: TTGGTAGGATTTTGTTGAGG |
| R: CAAGCTATTACGAAGCGCTC | |
| miR-18a | F: CTTATCGATCTATGC |
| R: CGCTATCTATGGACTCA | |
| CXCL13 | F: GTCCTAGCATCGAAGGCA |
| R: GTTCTACCTAGCTAGCTGG | |
| GAPDH | F: AGGTCGGTGTGAACGGATTTG |
| R: GGGGTCGTTGATGGCAACA | |
| U6 | F: ACCCTGAGAAATACCCTCACAT |
| R: GACGACTGAGCCCCTGATG | |
| RT-qPCR – reverse transcription quantitative polymerase chain reaction; SNHG15 – small nucleolar RNA host gene 15; miR – microRNA, CXCL13 – CXC chemokine ligand 13; GAPDH – glyceraldehyde-3-phosphate dehydrogenase; F – forward; R – reverse. |