16 August 2020: Animal Study
Extract EGb761 Attenuates Bleomycin-Induced Experimental Pulmonary Fibrosis in Mice by Regulating the Balance of M1/M2 Macrophages and Nuclear Factor Kappa B (NF-κB)-Mediated Cellular Apoptosis
Ling Pan AD* , Yuehong Lu BCE , Zhanhua Li BCEF , Yuping Tan BF , Hongmei Yang BF , Ping Ruan CDF , Ruixiang Li BFDOI: 10.12659/MSM.922634
Med Sci Monit 2020; 26:e922634
Table 1 List of primer sequences used in real-time polymerase chain reaction.
| Primers | Sequence (5′–3′) |
|---|---|
| Bax-F | AAGAAGCTGAGCGAGTGTCT |
| Bax-R | GTTCTGATCAGTTCCGGCAC |
| Bcl-2-F | GCCTTCTTTGAGTTCGGTGG |
| Bcl-2-R | CTCAGCCCAGACTCACATCA |
| Caspase-3-F | CAGCCAACCTCAGAGAGACA |
| Caspase-3-R | ACAGGCCCATTTGTCCCATA |
| Caspase-9-F | CAGGACCTTGGACAGTGACT |
| Caspase-9-R | AATGCCATCCAAGGTCTCGA |
| IL-10-F | GGTTGTCGTCTCATTCTGAAAGA |
| IL-10-R | GGTAGAGGACCCAAGTTCGTTAAGA |
| iNOS-F | CCCTTCAATGGTTGGTACATGG |
| iNOS-R | ACATTGATCTCCGTGACAGCC |
| GAPDH-F | CAACGGGAAACCCATCACCA |
| GAPDH-R | ACGCCAGTAGACTCCACGACAT |
| Bax – B-cell lymphoma-associated X protein; Bcl-2 – B-cell lymphoma-2; iNOS – inducible nitric oxide synthase; GAPDH – glyceraldehyde 3-phosphate dehydrogenase; F – forward; R – reverse. | |