25 January 2024 : Clinical Research
[In Press] Comprehensive Analysis of Immune Infiltration and Key Genes in Peri-Implantitis Using Bioinformatics and Molecular Biology Approaches
Qingxun Meng12ABCE, Jing Han21AEFG, Xi Zhang12CD, Wenxuan Su12BCF, Beibei Liu12BC, Taicheng Liu12BCDOI: 10.12659/MSM.941870
Med Sci Monit In Press; DOI: 10.12659/MSM.941870
Available online: 2024-01-25, In Press, Corrected Proof
Publication in the "In-Press" formula aims at speeding up the public availability of the pending manuscript while waiting for the final publication. The assigned DOI number is active and citable. The availability of the article in the Medline, PubMed and PMC databases as well as Web of Science will be obtained after the final publication according to the journal schedule
Abstract
BACKGROUND
Peri-implantitis is the main cause of failure of implant treatment, and there is little research on its molecular mechanism. This study aimed to identify key biomarkers and immune infiltration of peri-implantitis using a bioinformatics method.
MATERIAL AND METHODS
Three Gene Ontology (GO) gene expression profiles were selected from the Gene Expression Omnibus. Differentially expressed genes (DEGs) were identified by the LIMMA package, and functional correlations of DEGs were analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. Information on immune-related genes was obtained from ImmPort (https://www.immport.org) and InnateDB (http://www.innatedb.com). Immune-related DEGs were screened by least absolute shrinkage and selection operator (LASSO) and support vector machine-recursive feature elimination (SVM-RFE). The single-sample Gene Set Enrichment Analysis algorithm was used to analyze immune cell infiltration in gingival tissue between peri-implantitis and normal controls. Finally, results of bioinformatics analysis were verified by qPCR.
RESULTS
A total of 398 DEGs were identified, of which 96 were immune-related. Enrichment analysis showed these genes were enriched in inflammatory response, leucocyte chemotaxis, immune response-regulating signaling pathway, and cell activation. Seven key genes were selected by LASSO and SVM-RFE. Receiver operating characteristic curve results showed these genes had excellent diagnostic efficacy. Results of qPCR showed significant differences in the expression of these genes.
CONCLUSIONS
Differences in key genes and immune infiltration between peri-implantitis and gingival tissues of normal controls may provide new insights into the development of peri-implantitis. Elucidating the difference in immune infiltration between peri-implantitis tissues and normal tissues will help to understand the development of peri-implantitis.
Keywords: Peri-Implantitis; Computational Biology
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
08 May 2024 : Clinical Research
Effect of Individualized PEEP Guided by Driving Pressure on Diaphragm Function in Patients Undergoing Lapar...Med Sci Monit In Press; DOI: 10.12659/MSM.944022
21 Mar 2024 : Clinical Research
Impact of Serum Vitamin D, B6, and B12 and Cognitive Functions on Quality of Life in Peri- and Postmenopaus...Med Sci Monit In Press; DOI: 10.12659/MSM.943249
17 Mar 2024 : Clinical Research
Protective Role of TRPC3 Gene Polymorphism (rs10518289) in Obstructive Sleep Apnea Hypopnea Syndrome Among ...Med Sci Monit In Press; DOI: 10.12659/MSM.942667
27 Mar 2024 : Clinical Research
Impact of Patient Education Formats on Treatment Adherence and Denture Hygiene in Edentulous Patients: A Co...Med Sci Monit In Press; DOI: 10.12659/MSM.944175
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952