Hongshu Wang, Yong Liu, Shen Han, Yunfeng Zi, Yayong Zhang, Ruize Kong, Zu Liu, Zhibin Cai, Chongbin Zhong, Wei Liu, Lifeng Li, Lihong Jiang
Yan’an Hospital Affiliated to Kunming Medical University, Kunming, Yunnan, China (mainland)
Med Sci Monit 2020; 26:e925388
Available online: 2020-08-05
The protein NKX2-5 affects mammalian heart development. In mice, the disruption of Nkx2-5 has been associated with arrhythmias, abnormal myocardial contraction, abnormal cardiac morphogenesis, and death. However, the details of the mechanisms are unclear. This study was designed to investigate them.
MATERIAL AND METHODS: Rat cardiomyocytes from the H9c2 cell line were used in our study. First, we knocked down Nkx2-5 in the H9c2 cells and then validated consequent changes in cell proliferation and migration. We then used RNA sequencing to determine the changes in transcripts. Finally, we validated these results by quantitative reverse transcription-polymerase chain reaction.
RESULTS: We confirmed that Nkx2-5 regulates the proliferation and migration of H9c2 cells. In our experiments, Nkx2-5 regulated the expression of genes related to proliferation, migration, heart development, and disease. Based on bioinformatics analysis, knockdown of Nkx2-5 caused differential expression of genes involved in cardiac development, calcium ion-related biological activity, the transforming growth factor (TGF)-ß signaling pathway, pathways related to heart diseases, the MAPK signaling pathway, and other biological processes and signaling pathways.
CONCLUSIONS: Nkx2-5 may regulate proliferation and migration of the H9c2 cells through the genes Tgfb-2, Bmp10, Id2, Wt1, Hey1, and Cacna1g; rno-miR-1-3p; the TGF‑ß signaling pathway; the MAPK signaling pathway; as well as other genes and pathways.
Keywords: Heart Defects, Congenital, MAP Kinase Signaling System, Signal Transduction