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28 August 2020 : Clinical Research

Circ_cse1l Inhibits Colorectal Cancer Proliferation by Binding to eIF4A3

Bin Xu1ABCDEF, Ning Yang1BCDF, Yabin Liu1BCDF, Peng Kong2ACE, Mei Han2AG, Binghui Li1ABCDEFG*

DOI: 10.12659/MSM.923876

Med Sci Monit 2020; 26:e923876

Abstract

BACKGROUND: Circular RNAs (circRNAs) are involved in the growth of many tumors. However, the expression and possible role of circ_cse1l (hsa_circ_0060745) in colorectal cancer (CRC) are unclear. The present study was designed to explore the role of circ_cse1l in CRC.

MATERIAL AND METHODS: The levels of circ_cse1l expression in cancer tissues and serum samples of 50 patients with CRC and in control subjects were analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). CCK-8, colony formation, transwell and wound healing assays were performed to assess the functions of circ_cse1l in CRC cell lines after overexpression. The relationship between circ_cse1l and eIF4A3 during cell proliferation was analyzed by western blotting and RNA-binding protein immunoprecipitation (RIP).

RESULTS: qRT-PCR assays showed that the levels of expression of circ_cse1l were lower in CRC cell lines and in tissue and serum samples from patients with CRC than in control samples. The expression of circ_cse11 in CRC tissues had clinical significance, as its level of expression was inversely associated with the depth of tumor invasion. Overexpression of circ_cse1l in HT29 and HCT116 cells markedly reduced cell proliferation and metastasis. Western blotting showed that circ_cse1l overexpression dowregulated the expression of PCNA protein. RIP results demonstrated that circ_cse1l inhibited the proliferation of CRC cells by binding to eIF4A3.

CONCLUSIONS: The expression of circ_cse1l is downregulated in CRC. Furthermore, circ_cse1l downregulated PCNA expression by binding to eIF4A3, inhibiting the proliferation of CRC cells.

Keywords: Biological Markers, Colorectal Neoplasms, Diagnosis, DNA, Circular, Eukaryotic Initiation Factor-4A

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750