03 June 2020 : Laboratory Research
The Effect of Shen Qi Wan Medicated Serum on NRK-52E Cells Proliferation and Migration by Targeting Aquaporin 1 (AQP1)
Ying He12ABCEF, Yu Ting Bao1ABCE, Hong Shu Chen3ABCF, Yi Tao Chen1ADE, Xiao Jie Zhou1BCDE, Yuan Xiao Yang4ACE, Chang Yu Li1AG*DOI: 10.12659/MSM.922943
Med Sci Monit 2020; 26:e922943
Abstract
BACKGROUND: Shen Qi Wan (SQW) as a well-known formula for the amelioration of kidney yang deficiency syndrome (KYDS), and it has been widely employed in traditional Chinese medicine (TCM). This study aimed to investigate the effect and underlying mechanism of SQW medicated serum on proliferation and migration in NRK-52E cells.
MATERIAL AND METHODS: We employed the real-time cell analysis (RTCA) system to investigate the effect of SQW medicated serum on proliferation and migration in NRK-52E cells. In addition, the migration was further investigated by using a wound-healing assay. The mRNA and protein expression level of aquaporin 1 (AQP1) of NRK-52E cells with SQW medicated serum-treated were quantified by real-time quantitative polymerase chain reaction (q-PCR) and western blot assay, respectively. Furthermore, NRK-52E cells were transfected with lentivirus AQP1-RNAi to assess migratory cell abilities in vitro.
RESULTS: The migratory abilities of NRK-52E cells were significantly increased after SQW medicated serum treatment (P<0.05), and no significant difference in cell proliferation. In addition, SQW medicated serum was significantly upregulated the mRNA and protein expression level of AQP1 in NRK-52E cells (P<0.05). Additionally, the in vitro metastasis test proved that knockdown of AQP1 suppressed migratory abilities according to RTCA and wound healing test while was reversed by SQW medicated serum (P<0.05).
CONCLUSIONS: Our study demonstrates that SQW medicated serum effectively promotes the migration of NRK-52E cells by increasing AQP1 expression, and AQP1 may be as a therapeutic target of SQW for renal injury treatment under KYDS.
Keywords: Aquaporin 1, endothelial cells, Cell Line, Drugs, Chinese Herbal, Kidney, Kidney Diseases, Molecular Targeted Therapy, RNA, Messenger, Yang Deficiency
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