08 June 2020 : Laboratory Research
Suppression of Circular RNA Hsa_circ_0109320 Attenuates Non-Small Cell Lung Cancer Progression via MiR-595/E2F7 AxisQiaohong Bai1ABEFG*, Li Li2BCD, Futao Chen3B, Jiang Zhu3B, Lifeng Cao3F, Yang Yang3E, Fukuan Zhong3F
Med Sci Monit 2020; 26:e921200
BACKGROUND: Circular RNAs (circRNAs) are frequently aberrantly expressed in non-small cell lung cancer (NSCLC) and are considered to exert a pivotal role in the occurrence and development of NSCLC via targeting and negatively regulating microRNAs (miRNAs). We aimed to investigate the role of hsa_circ_0109320 in the proliferation, invasion and apoptosis of NSCLC, and explore its underlying molecular mechanism.
MATERIAL AND METHODS: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis was performed to determine the circ_0109320 and miR-595 expression in tissues or cells. Western blot analysis was conducted to examine the cleaved caspase-3, Bax, Bcl-2, and E2F7 protein expression. Transwell detection was used to evaluate the invasion level of NSCLC cell lines.
RESULTS: The results of present study indicated that circ_0109320 expression in NSCLC patients was upregulated significantly in tumor tissues compared with tissues adjacent to carcinoma. Upregulated circ_0109320 level was significantly associated with TNM stages as well as lymph node metastasis of NSCLC. Moreover, downregulation of circ_0109320 attenuated proliferation and invasion while promoting apoptosis in NSCLC cells. We further confirmed that circ_0109320 could sponge miR-595 to upregulate E2F7 expression. Silencing of miR-595 or overexpression of E2F2 could partially reversed the inhibitory role of circ_0109320 knockdown in NSCLC cells. These data provided evidence that the suppression of circ_0109320 attenuates NSCLC cell proliferation and invasion and enhances apoptosis through the miR-595/E2F7 pathway.
CONCLUSIONS: Circ_0109320/miR-595/E2F2 axis may exert a pivotal role in the pathological mechanism of NSCLC progression, and it has potential application in the future treatment of NSCLC.
Keywords: Carcinoma, Non-Small-Cell Lung, E2F2 Transcription Factor, MicroRNAs, RNA, Untranslated, Adult, Aged, Aged, 80 and over, Apoptosis, Apoptosis Regulatory Proteins, Cell Proliferation, Disease Progression, Gene Expression Regulation, Neoplastic, Lung Neoplasms, Male, Middle Aged, Neoplasm Invasiveness, RNA, RNA, Circular, Real-Time Polymerase Chain Reaction
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