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29 January 2020 : Animal Research  

Evaluation of 2 Rat Models for Sepsis Developed by Improved Cecal Ligation/Puncture or Feces Intraperitoneal-Injection

Hui Fang1ABCDEF, Chenchen Gong1BCEF, Jianyu Fu2ABCEF, Xu Liu2ADF, Hongying Bi2BCF, Yumei Cheng2BE, Yiyuan Liu2BF, Yan Tang2AEF*, Difen Wang2BF

DOI: 10.12659/MSM.919054

Med Sci Monit 2020; 26:e919054

Abstract

BACKGROUND: The aim of this study was to evaluate the clinical characteristics of 2 rat models of sepsis for improved cecal ligation/puncture (CLP) and feces intraperitoneal-injection (FIP), including systemic inflammation, organ dysfunction, and blood coagulation.

MATERIAL AND METHODS: Sixty-two male SD rats were randomly divided into 3 groups: a normal control group (NC, n=6), a CLP group (n=28), and a FIP group (n=28). Ten rats each in the CLP and FIP groups were observed for 72-h mortality rate. The remaining 18 rats in each group were divided into 3 subgroups (n=6) according to their post-operation period (6, 12, and 24 h). Abdominal arterial blood was collected to determine the lactic acid (Lac) concentration, prothrombin time (PT), active partial prothrombin time (APTT), plasmic interleukin-6 (IL-6) level, and cardiac troponin (cTnI) level. The intestines, lung, and heart were collected for pathological examination.

RESULTS: The 72-h mortality rates in the CLP and FIP groups were 60% and 100%, respectively. The Lac level in both groups was significantly elevated at 6, 12, and 24 h after modeling. Compared with the NC group, PT in the CLP and FIP groups was prolonged at 12 and 24 h, and APTT was significantly prolonged at 6 h. IL-6 levels in the CLP and FIP groups peaked at 6 h. The cTnI level in the FIP group was significantly higher at 12 h after modeling compared with the NC group. The intestines, lung, and heart were pathologically damaged at 6 h, and this damage worsened over time.

CONCLUSIONS: Both modeling methods induced sepsis in rats and closely mimicked the clinical conditions, but FIP was easier to establish and was more suitable for standardization.

Keywords: Cytokines, Multiple Organ Failure, Sepsis, Cecum, Disease Models, Animal, Feces, Injections, Intraperitoneal, Interleukin-6, Intestines, Lactic Acid, Ligation, Male, Myocardium, Prothrombin Time, Punctures, Rats, Sprague-Dawley, Survival Analysis, troponin I

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750