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22 October 2019 : Laboratory Research  

Effect of Netrin-1 Anti-Inflammatory Factor on Acute Lung Injury in Sepsis Rats

Jisong Liu12CDEF, Juan Du3BCF, Xiu Cheng4CD, Xiangzhou Zhang2BC, Yong Li2BC, Xiujun Fu5DF, Xulin Chen1AG*

DOI: 10.12659/MSM.917279

Med Sci Monit 2019; 25:7928-7935

Abstract

BACKGROUND: Acute lung injury (ALI) often occurs early and seriously in the progress of sepsis. Netrin-1 is demonstrated to be an effective anti-inflammatory agent. However, whether netrin-1 can relieve sepsis-induced ALI remains unknown.

MATERIAL AND METHODS: The sepsis rat model was built with the method of cecal ligation and puncture (CLP). The lung tissue changes were represented as the results of hematoxylin-eosin (HE) staining, wet-to-dry (W/D) ratio, Western blot analysis, and immunohistochemistry. An in vitro lung injury model was simulated with LPS-induced BEAS-2B cells. The cell transfection effects were evaluated by Western blot analysis and RT-qPCR analysis. TNF-α, IL-1β, and IL-6 levels were detected by Western blot analysis in LPS-induced BEAS-2B cells.

RESULTS: Obvious inflammation caused by sepsis appeared in lung tissues with the increase of the W/D ratio and expression of inflammatory cytokines. Netrin-1 and its receptor UNC5B were reduced in sepsis. However, upregulation of netrin-1 alleviated the levels of inflammation and increased the UNC5B levels in BEAS-2B cells.

CONCLUSIONS: Netrin-1 protects against ALI in sepsis rats through its anti-inflammation effect and may provide a novel treatment to prevent lung injury caused by sepsis.

Keywords: acute lung injury, exosomes, Sepsis, Anti-Inflammatory Agents, Bronchoalveolar Lavage Fluid, Cytokines, Lung, Netrin-1

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750