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Medical Science Monitor Basic Research


eISSN: 1643-3750

α-Pinene Induces Apoptotic Cell Death via Caspase Activation in Human Ovarian Cancer Cells

Jing Hou, Yi Zhang, Yanju Zhu, Bingfeng Zhou, Chunli Ren, Shuang Liang, Yanwei Guo

Department of Obstetrics, Affiliated Hospital of Chengde Medical University, Chengde, Hebei, China (mainland)

Med Sci Monit 2019; 25:6631-6638

DOI: 10.12659/MSM.916419

Available online: 2019-09-04

Published: 2019-09-04


BACKGROUND: The plant-derived terpenoid, alpha-pinene is a bicyclic monoterpene potentially useful for the treatment of various diseases which also includes cancer and its types. The present investigation is about finding the anticancer activity of the alpha-pinene extracted from the leaves of Boswellia dalzielii over the PA-1 cancer cells of the human ovary.
MATERIAL AND METHODS: The cytotoxic activity of the alpha-pinene was evaluated using MTT and LDH assays which indicated that alpha-pinene could induce cytotoxicity in cancer-causing cells in the ovary. The consequences of alpha-pinene on the cell sequence regulation were determined by the staining technique using propidium iodide (PI) followed with flow cytometry.
RESULTS: The cell cycle distribution analysis showed that alpha-pinene inhibit the cycle progression from G₂ to M phase. In addition, apoptosis analysis is done through the double staining investigation using Annexin V-FITC/PI to analyze the controlled growth of alpha-pinene which is associated with the apoptosis. Caspase-3 a crucial enzyme involved in apoptosis was markedly increased in the a-pinene treated PA-1 cells. The apoptosis results reveal, that the cancer cells at the human ovary with alpha-pinene induces the significant populations of apoptotic cells.
CONCLUSIONS: Overall, alpha-pinene may exert anticancer effects in PA-1 cells by promoting cytotoxicity, suppression of cell sequence progression along with the programmed cell death.

Keywords: Cytotoxicity Tests, Immunologic