12 August 2019 : Laboratory Research
Wogonin Increases Cisplatin Sensitivity in Ovarian Cancer Cells Through Inhibition of the Phosphatidylinositol 3-Kinase (PI3K)/Akt PathwayFeng Xing1ABCEF, Cong Sun2BCDE, Ning Luo1BC, Yuanying He1BC, Mengmeng Chen1DF, Siyu Ding1BC, Chenghua Liu1BC, Lijin Feng3EF, Zhongping Cheng1A*
Med Sci Monit 2019; 25:6007-6014
BACKGROUND: Wogonin (5,7-dihydroxy-8-methoxyflavone), one of flavonoids isolated from the Scutellaria baicalensis, has been regarded as an anticancer candidate because of its maximal efficacy in cancer cells. This study aimed to explore the possible mechanism that wogonin uses to enhance the sensitivity of ovarian cancer cells to cisplatin chemotherapy.
MATERIAL AND METHODS: The growth inhibition rates of ovarian cancer cells SKOV3/DDP and C13* were assessed by Cell Counting Kit-8 (CCK-8) assay. The apoptosis was assessed under a fluorescence microscope following staining with Hoechst. We further analyzed the expression of Bcl-2, cleaved caspases-3, cleaved-PARP, and phospho-Akt by western blotting.
RESULTS: In the present study, we found that wogonin reduced proliferation of ovarian cancer cells SKOV3, SKOV3/DDP, OV2008, and C13* in dose- and time-dependent manners and it sensitized cisplatin-induced cytotoxicity. Moreover, treatment with wogonin also increased cisplatin-resistant SKOV3/DDP and C13* cells to low dose cisplatin-induced cell apoptosis. Additionally, such treatment resulted in a significant decrease in phosphorylated Akt.
CONCLUSIONS: Wogonin could significantly increase the sensitivity of cisplatin-resistant ovarian cancer cells to cisplatin by downregulating the PI3K/Akt pathway.
Keywords: Antineoplastic Agents, Cisplatin, Ovarian Neoplasms, Apoptosis, Carcinoma, Ovarian Epithelial, Cell Proliferation, Drug Resistance, Neoplasm, Flavanones, Ovary, phosphatidylinositol 3-kinase, Phosphatidylinositol 3-Kinases, Proto-Oncogene Proteins c-akt
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