19 February 2019 : Laboratory Research
CircRNA circ_0067934 Overexpression Correlates with Poor Prognosis and Promotes Thyroid Carcinoma ProgressionHuihui Wang1B, Xiaoguang Yan1BC, Haijun Zhang1BCD, Xiaorong Zhan2AB*
Med Sci Monit 2019; 25:1342-1349
BACKGROUND: Circular RNAs are important regulators in human cancers, including thyroid carcinoma. The circ_0067934 RNA is reported to participate in hepatocellular carcinoma, esophageal squamous cell carcinoma, and lung cancer. Whether it regulates thyroid carcinoma remains unclear. The purpose of this study was to research potential mechanisms of circ_0067934 in thyroid tumors to provide potential new diagnostic and treatment targets.
MATERIAL AND METHODS: The expression level of circ_0067934 in thyroid tumors, adjacent tissues, and cell lines was measured by qRT-PCR. The Kaplan-Meier survival curve analysis was used to explore the relationship between circ_0067934 level and survival time of patients. Circ_0067934 was knocked down to research its functional role in thyroid tumors. Cell proliferation was detected by CCK-8 (cell counting kit-8) assay. Migration and invasion were analyzed by Transwell assay. Western blot was applied to analyze the expression of epithelial-mesenchymal-transition (EMT) and PI3K/AKT related proteins.
RESULTS: Compared with adjacent tissue, circ_0067934 was highly expressed in thyroid tumors. Circ_0067934 expression level was highly expressed in thyroid tumor cell lines. Patients with high expression of circ_0067934 showed lower survival rates. Knockdown of circ_0067934 inhibited cell proliferation, migration, and invasion and also promoted apoptosis. In addition, circ_0067934 knockdown inhibited EMT and PI3K/AKT signaling pathways.
CONCLUSIONS: circ_0067934 could improve the development of thyroid carcinoma by promoting EMT and PI3K/AKT signaling pathways.
Keywords: Phosphatidylinositol 3-Kinases, RNA, Untranslated, Adult, Cell Movement, Cell Proliferation, China, Disease Progression, Epithelial-mesenchymal transition, Gene Expression Regulation, Neoplastic, Kaplan-Meier Estimate, Male, Middle Aged, Neoplasm Invasiveness, Prognosis, Proportional Hazards Models, Proto-Oncogene Proteins c-akt, RNA, RNA, Circular, Signal Transduction, transcriptome
01 May 2022 : EditorialEditorial: Cardiovascular Complications at One Year After SARS-CoV-2 Infection are Independent of Underlying Cardiovascular Risk Factors or Severity of COVID-19
Med Sci Monit 2022; 28:e937048
04 May 2022 : Clinical ResearchEffects of Wearing Face Masks on Exercise Capacity and Ventilatory Anaerobic Threshold in Healthy Subjects ...
Med Sci Monit In Press; DOI: 10.12659/MSM.936069
22 April 2022 : Clinical ResearchFactors Associated with Falls During Hospitalization for Coronavirus Disease 2019 (COVID-19)
Med Sci Monit In Press; DOI: 10.12659/MSM.936547
27 April 2022 : Meta-AnalysisEffect of the COVID-19 Pandemic on Serum Vitamin D Levels in People Under Age 18 Years: A Systematic Review...
Med Sci Monit In Press; DOI: 10.12659/MSM.935823
19 May 2022 : Clinical ResearchAssociation Between Serum Homocysteine Levels and Severity of Diabetic Kidney Disease in 489 Patients with ...
Med Sci Monit In Press; DOI: 10.12659/MSM.936323
18 May 2022 : Clinical ResearchPhysical and Psychosocial Concept Domains Related to Health-Related Quality of Life (HRQL) in 50 Girls and ...
Med Sci Monit In Press; DOI: 10.12659/MSM.936801
18 May 2022 : Clinical ResearchAssociation Between Variants of the Mannose-Binding Lectin 2 Gene and Susceptibility to Sepsis in the Haina...
Med Sci Monit In Press; DOI: 10.12659/MSM.936134
Most Viewed Current Articles
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
01 Nov 2020 : Review articleLong-Term Respiratory and Neurological Sequelae of COVID-19
Med Sci Monit 2020; 26:e928996