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10 January 2019 : Animal Research  

The Combined Use of Tea Polyphenols and Lactobacillus Plantarum ST8SH Bacteriocin in a Rabbit Model of Infection Following Femoral Fracture with Internal Fixation

Zhonghua Xu12CDE, Jin Li3CDE, Xindie Zhou1BCD, Jianbo Dai4CDE, Junjie Zhang1CDE, Yong Huang5CD, Nanwei Xu1ABC*

DOI: 10.12659/MSM.912971

Med Sci Monit 2019; 25:312-317

Abstract

BACKGROUND: Worldwide, the increasing use of antibiotics has resulted in antimicrobial resistance, leading to studies to find alternative antimicrobial treatments. Tea polyphenols have antibacterial properties. Bacteriocins produced by probiotic lactobacilli can inhibit Gram-positive bacteria. This study used a rabbit model of infection, following femoral fracture with internal fixation, to evaluate the efficacy of the combined use of tea polyphenols and Lactobacillus plantarum ST8SH bacteriocin.

MATERIAL AND METHODS: Twenty-four New Zealand White rabbits underwent femoral fracture, internal fixation, and insertion of a mini-titanium implant, and were inoculated intravenously with suspensions of Staphylococcal bacteria. Four treatment groups included group A, injected with tea polyphenols and bacteriocins (N=6); group B, injected with cefradine and bacteriocins (N=6); group C, injected with tea polyphenols and cefradine (N=6); and group D (controls), injected with saline (N=6). Blood samples were collected at 1, 6, 12, 24, and 48 hours after the injection of bacteriocins. Biofilms that formed on the mini-titanium implant were studied by fluorescence microscopy. Serum levels of level of interleukin (IL)-8, IL-6, and tumor necrosis factor-α (TNF-α) were measured using an enzyme-linked immunosorbent assay (ELISA).

RESULTS: The combination of tea polyphenols and bacteriocins (group A) had a significant inhibitory effect on Staphylococcus aureus (P<0.05) and significant differences in serum levels of IL-8, TNF-α, and IL-6 levels in serum (P<0.05) when compared with groups, B, C, and D.

CONCLUSIONS: In a rabbit model of femoral fracture with internal fixation, the combined use of tea polyphenols and Lactobacillus plantarum ST8SH bacteriocin effectively controlled Staphylococcus aureus infection.

Keywords: Anti-Infective Agents, Biofilms, polyphenols, Anti-Bacterial Agents, Bacteriocins, Cytokines, Disease Models, Animal, Femoral Fractures, Fracture Fixation, Internal, infections, Interleukin-8, Lactobacillus plantarum, Rabbits, Staphylococcus aureus, Tea, Tumor Necrosis Factor-alpha

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750