The Flavanone, Naringenin, Modifies Antioxidant and Steroidogenic Enzyme Activity in a Rat Model of Letrozole-Induced Polycystic Ovary Syndrome
Yanli Hong, Yanyun Yin, Yong Tan, Ke Hong, Huifang Zhou
Department of Reproductive Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China (mainland)
Med Sci Monit 2019; 25:395-401
Available online: 2019-01-13
Worldwide, polycystic ovary syndrome (PCOS) is a prevalent endocrine and metabolic disorder that affects women of reproductive age. Naringenin is a natural flavanone, derived from grapefruit. The aims of this study were to investigate the effects of naringenin on the antioxidant and steroidogenic enzyme activity in a rat model of letrozole-induced PCOS.
MATERIAL AND METHODS: The induction of PCOS was undertaken by giving 28 female Sprague-Dawley rats a dose of letrozole (1 mg/kg) daily for 21 days. There were four treatment groups: Group I (n=7) received 1% of carboxymethyl cellulose (CMC); Group II (n=7) received 1% CMC plus naringenin 20 mg/kg; Group III (n=7) received letrozole only; Group IV (n=7) received letrozole plus naringenin 20 mg/kg. Estradiol, testosterone, and steroidogenic enzyme activities, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activity were determined in the four treatment groups, and histology was performed on the rat ovarian tissue. Serum glucose levels were measured with a glucometer.
RESULTS: Naringenin treatment in a rat model of PCOS significantly increased the levels of the reactive oxygen species (ROS) scavenging enzymes CAT, SOD, and GPX (p<0.05), and prevented weight increase. Naringenin treatment resulted in a significant reduction in serum glucose levels (p<0.05), normalized estradiol and testosterone levels steroidogenic enzyme activity, and maintained the normal anatomy of the ovaries.
CONCLUSIONS: Naringenin treatment, in a rat model of PCOS, demonstrated antioxidant and steroidogenic enzyme activity.
Keywords: Enzyme Activation, Gene Expression, Polycystic Ovary Syndrome