15 April 2019 : Animal Research
Apigenin Alleviates Myocardial Reperfusion Injury in Rats by Downregulating miR-15b
PeiPei Wang1ABCEFG*, Jian Sun1ABC, SuJun Lv2BCD, Tao Xie3ABCD, XueDan Wang1BCDDOI: 10.12659/MSM.912014
Med Sci Monit 2019; 25:2764-2776
Abstract
BACKGROUND: We investigated whether apigenin could mitigate myocardial reperfusion injury in rats, and a possible mechanism was proposed.
MATERIAL AND METHODS: The I-R injury model was established in rats along with a sham group as control, and the expressions of microRNA-15b (miR-15b), JAK2, and p-JAK2 in the myocardia of the 2 groups were detected. Apoptosis and reactive oxygen species (ROS) were also detected. Rats in the I-R injury model were divided into 3 groups in vivo: the 1I-R group, the 2I-R+solvent group, and the 3I-R+apigenin group. Expression of miR-15b, JAK2, p-JAK2, STAT3, and p-STAT3 in the myocardia of the 3 groups were detected. ROS content, apoptosis, MDA content, SOD, and CAT activities were detected. Rat myocardial H9C2 cells were cultured in vitro and divided into 5 treatment groups in vitro; expressions of miR-15b, JAK2, p-JAK2, STAT3, and p-STAT3 in H9C2 cells were detected, and the apoptosis and ROS content were detected by flow cytometry.
RESULTS: We found that the increased miR-15b expression during myocardial I-R injury in rats downregulated the expression of JAK2 and activity of the JAK2-STAT3 pathway, promoted myocardial apoptosis and ROS production, and aggravated myocardial I-R injury. Apigenin treatment can downregulate miR-15b expression, increase the expression of JAK2 and the activity of JAK2-STAT3 pathway, reduce myocardial apoptosis and ROS production, and alleviate myocardial I-R injury.
CONCLUSIONS: Api treatment downregulated the expression of miR-15b and upregulated the expression of JAK2 and the activity of the JAK2-STAT3 pathway, thereby alleviating myocardial I-R injury, cardiomyocyte apoptosis, and ROS production in vitro.
Keywords: Apigenin, Myocardium, Down-Regulation, Janus Kinase 2, Myocardial Reperfusion Injury
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