Guiyun Ren, Yanning Zhang, Jie Wang, Huijuan Liu, Fusheng Dong
Department of Oral and Maxillofacial Surgery, College and Hospital of Stomatology, Hebei Medical University; The Key Laboratory of Stomatology, Shijiazhuang, Hebei, China (mainland)
Med Sci Monit 2018; 24: ANS3772-3781
Available online: 2018-06-05
Salivary pleomorphic adenoma is one of the most common salivary gland tumors. It has a relatively high tendency to recur and a high risk of malignant transformation. The present study aimed to study the effect of XT-I gene silencing on the implanting growth of salivary pleomorphic adenoma.
MATERIAL AND METHODS: Primary cultures of SPA cells and fibroblasts from the same patient were assessed. The adenovirus vector Ad-shRNA-XT-I was constructed and transfected into SPA cells. The expression of XT-I gene and XT-I protein was detected by real-time PCR and Western blot. The contents of proteoglycans were detected. The SPA cells transfected with Ad-shRNA-XT-I (group SPA-XT-I) and Ad-shRNA-HK (group SPA-HK), as well as without transfection (group SPA), were implanted into ADM scaffold with fibroblasts and then transferred into 18 BALB/C-nu nude mice for 3 months.
RESULTS: Primary cultures showed SPA cells were positive for human CK and S-100 protein and the fibroblasts were positive for human vimentin. The expressions of XT-I gene and protein were decreased by 51% and 51.31%, respectively. The content of proteoglycans was reduced by 48.45%. The results of the implanting growth in vitro and in vivo of nude mice indicated that no tumors grew in the SPA-XT-I group, whereas SPA grew in groups SPA-HK and SPA positive for human a-SMA, S-100 protein, and calponin.
CONCLUSIONS: XT-I gene silencing effectively inhibited the implanting growth of SPA.
Keywords: Adenoma, Pleomorphic, Proteoglycans, RNA Interference, Tissue Engineering