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eISSN: 1643-3750

Astrocyte Elevated Gene 1 (AEG-1) Acts as a Promoter Gene in Clear Cell Renal Cell Carcinoma Cell Growth and Metastasis

Da Peng Yu, Yan Zhou

Department of Surgical Urology, The First People’s Hospital of Jining City, Jining, Shandong, China (mainland)

Med Sci Monit 2018; 24:8213-8223

DOI: 10.12659/MSM.911010

Available online:

Published: 2018-11-15

BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is usually incurable once it progresses to metastatic stage. Hence, in-depth investigations to reveal the precise molecular mechanisms behind the metastasis of ccRCC are required to improve the therapeutic outcome of ccRCC.
MATERIAL AND METHODS: The level of astrocyte elevated gene 1 (AEG-1) in ccRCC tissues and cell lines was determined by quantitative real-time PCR (qRT-PCR) assay. The MTS, colony formation, wound-healing, and Transwell invasion assays were used to assess the role of AEG-1 in ccRCC cells growth, migration, and invasion in vitro, respectively. Xenograft model and lung metastasis models were constructed to analyze the functions of AEG-1 in ccRCC cells growth and metastasis in vivo.
RESULTS: We found that AEG-1 was overexpressed in ccRCC and was associated with the progression of ccRCC. Knocked-down AEG-1 impaired the migration and invasion of ccRCC cells in vitro. Furthermore, under-expression of AEG-1 caused complete inhibition of ccRCC cells growth and metastasis in vivo. In contrast, overexpression of AEG-1 significantly increased the migration and invasion ability of ccRCC cells in vitro. Finally, we revealed that AEG-1 boosted the metastatic ability of ccRCC cells via regulating Notch homolog 1 (Notch1).
CONCLUSIONS: The AEG-1/Notch1 signaling axis plays a vital role in ccRCC cell growth and metastasis.

Keywords: Carcinoma, Renal Cell, Neoplasm Metastasis, Receptor, Notch1